SOLID PHASE EXTRACTION (SPE)
TRAINING COURSE
Solid phase extraction (SPE) offers a highly quantitative, sensitive and robust approach to removing matrix interference from complex samples. With low setup cost, ease of use and potential for high throughput and automation, this efficient and accessible technique provides a solution to many challenging sample types. These benefits, however, rely on well-developed methods requiring a high level of understanding of this multi-step preparation technique, matched with good experimental design from the development analyst.
Our comprehensive course is designed to increase the expertise required to develop robust methods and use these efficiently in a routine environment. We work through foundation principles then learn to apply these to relevant practical applications, discussing how resolve common challenges in routine analysis and method development.
SOLID PHASE EXTRACTION (SPE) COURSE DETAILS
- The need for sample preparation
- The range of techniques available for sample clean-up
- Liquid/liquid Extraction (LLE) and Supported Liquid Extraction (SLE)
- QuEChERS
- Phospholipid Removal
- The basics of SPE and practical considerations
- Sorbent Selection, retention mechanisms and generic protocols
- What are the various extraction techniques offered by SPE
- Method development and protocol troubleshooting
- Instrumentation considerations
We offer flexible delivery options for this instructor-led course:
- On-site: one day at your lab or location of your choice.
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Online: two half days.
View our online training calendar for upcoming dates.
No previous experience is needed for this course but an understanding on LC and GC analysis and method development would be beneficial.
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Liquid-liquid Extraction and Supported liquid-liquid extraction
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Analyte and Matrix Trapping
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SPE –Physical Parameters – tube size, sorbent type, bed weight
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SPE Protocol
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SPE Modes – non-polar and polar SPE
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Ion Exchange SPE – cation, anion and strong / weak modes
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Mixed Mode SPE
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Internal Standards
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Method Development
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Method Troubleshooting
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Overview of 96-well technology (optional)
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Solid Phase Microextraction (SPME) (optional)
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QuEChERS (optional)
