YMC-Pack Pro C4 HPLC Columns

YMC-Pack Pro C4 Analytical HPLC Columns

With Pro C4, YMC offers a stationary phase based on the well-known ultra pure silica of the ProFamily. Compared to a C18 phase with the same eluent, C4 phases are less hydrophobic and gives shorter retention times for non-polar compounds while the retention time of polar analytes are virtually unaffected. This makes the Pro C4 an interesting alternative especially when short analysis times are required.

The YMC-Pack Pro C4 columns offer a unique balance of hydrophobicity and speed, making them an excellent choice for fast chromatography of polar organic molecules, particularly peptides and small proteins. Based on YMC’s high-purity silica, these columns are tailored to provide efficient separation with reduced retention times compared to more hydrophobic phases.

Part Number Product Description Price Qty
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Features

  • C4 stationary phase with USP L26 desingation
  • Designed with shorter butyl groups to provide less hydrophobic interactions than C8 or C18 columns
  • Delivers faster elution of non-polar compounds while maintaining adequate retention for polar analytes
  • Proprietary endcapping technology minimises the effect of residual silanols on peak shapes, ensuring symmetrical peaks for basic compounds
  • Ideal for applications requiring quick run times without sacrificing separation quality, particularly valuable in high-throughput environments
  • Suited for the rapid analysis of peptides, small proteins, and other polar organic compounds in pharmaceutical and biochemical research
  • Available with ultra-pure fully porous particles in 3 µm and 5 µm sizes
  • Fully scalable up to preparative dimensions
  • Guard columns recommended to increase the analytical column’s lifetime

Specifications

  YMC-Pack Pro C4
Bonded Phase C4
Particle Type Fully Porous
Particle Size 3 µm, 5 µm
Pore Size 120 Å
Surface Area 330 m2/g
Endcapped Yes
Carbon Load 7%
Maximum Temperature 50°C
pH Range 2 - 7.5
Hardware Stainless Steel
USP Designation L26

Literature

Full details on the YMC classic range of HPLC columns
YMC columns for reversed phase HPLC and UHPLC