Advion-Interchim puriFlash 5.250P

The puriFlash 5.250P system from Advion-Interchim Scientific is a purification system designed specifically for the complex separation of peptides and oligonucleotides. The system’s low dead volume, combined with compatibility for small-particle columns, enables high-resolution separation of peptides with closely related amino acid sequences and distinguish oligonucleotides in terms of sequence.

Tailored for both flash and preparative chromatography, the 5.250P integrates an optical UV cell with a 1.3 mm path length, allowing for enhanced detection sensitivity, even for low-concentration analytes or those with weak UV absorbance. Multiple detection options –including UV, ELSD, and mass spectrometry – ensure comprehensive monitoring and recovery of target compounds throughout the purification process.

The system is designed with operational safety and sample integrity in mind. It includes automated solvent level control with run-pausing functionality to protect columns from drying, as well as multiple strategically placed leak detectors to safeguard against sample loss. The elution pump features a wash disc system and integrated micro-pump, allowing reliable use of buffered solvents without the risk of salt crystallisation on pump pistons.

System operation is managed via ClearPeak software, displayed on a 15 inch touchscreen interface with full stylus and fingertip control. Genius, the software’s AI assistant, supports intuitive method configuration and remote monitoring, allowing users to develop gradients from TLC or HPLC data and manage runs from outside the laboratory.

Designed for efficiency in compact environments, the puriFlash 5.250P supports the integration of a fume encloser, freeing valuable fume hood space for synthesis work. Combining advanced automation, high-resolution detection, and integrated safety measures, it is ideally suited to laboratories handling biomolecules.