HPLC Solvents from Agilent

Agilent InfinityLab HPLC gradient grade solvents have been developed for optimum performance with Agilent LC instruments and applications. All solvents are manufactured with the lowest levels of impurities, and are pre-filtered through a 0.2 µm filter to protect your system from contaminants and clogging. Benefits include reduced troubleshooting and instrument downtime caused by impure solvents. Agilent HPLC solvents are easy to use, and offer excellent lot-to-lot reproducibility, ensuring consistent results.

Agilent InfinityLab HPLC solvents are shipped in 2.5 L high quality amber borosilicate glass bottles to preserve solvent integrity. Batch specific certificates of analysis are also available to meet GLP specific requirements.

Agilent offers HPLC gradient grade acetonitrile, methanol and water solvents, which are widely used in reversed phase HPLC applications.

Part Number Product Description Price Qty
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Features

  • Pre-filtered at 0.2 μm to safeguard your system from contaminants and clogging
  • Maintains the lowest impurity levels to minimise ghost peaks in gradient runs
  • Improves column and capillary lifespan, promoting cost efficiency
  • Excellent lot-to-lot reproducibility ensures consistent results
  • Designed and optimised for seamless integration with Agilent instruments
  • Shipped in high quality amber borosilicate glass bottles to preserve solvent integrity
  • 2.5 L bottles for convenience and ample supply

HPLC Acetonitrile:

  • Synonyms: Cyanomethane, methyl cyanide, ACN, ethyl nitrile
  • Formula: CH3CN
  • CAS number: 75-05-8
  • Molecular weight: 41.05 g/mol

HPLC Methanol:

  • Synonym: Methyl alcohol
  • Formula: CH3OH
  • CAS number: 67-56-1
  • Molecular weight: 32.04 g/mol

HPLC Water:

  • Synonym: De-ionised water
  • Formula: H2O
  • CAS number: 7732-18-5
  • Molecular weight: 18.02 g/mol

Specification

The below information outlines the key parameters and limits for Agilent’s HPLC gradient grade solvents, highlighting their minimal impurity levels.

These specifications demonstrate that InfinityLab solvents are the ideal choice for use with LC instruments, ensuring optimal performance.

 

HPLC Acetonitrile Specifications:

Identity (by Infrared Spectroscopy): Identity confirmed Fluorescence (as quinine at 245 nm): ≤ 1.0 ppb
Purity (by Gas Chromatography): ≥ 99.9% Fluorescence (as quinine at 365 nm): ≤ 0.5 ppb
Gradient Suitability (at 210 nm): ≤ 1.0 mAU Residue on Evaporation: ≤ 2.5 ppm
Gradient Suitability (at 254 nm): ≤ 0.5 mAU Water: ≤ 0.02%
Absorbance (at 195 nm): ≤ 0.097 AU Colour: Colourless
Absorbance (at 210 nm): ≤ 0.040 AU Acidity: ≤ 0.2 µeq/g
Absorbance (at 225 nm): ≤ 0.010 AU Alkalinity: ≤ 0.2 µeq/g

 

HPLC Methanol Specifications:

Identity (by Infrared Spectroscopy): Identity confirmed Fluorescence (as quinine at 245 nm): ≤ 1.0 ppb
Purity (by Gas Chromatography): ≥ 99.9% Fluorescence (as quinine at 365 nm): ≤ 0.5 ppb
Gradient Suitability (at 235 nm): ≤ 2.0 mAU Residue on Evaporation: ≤ 2.5 ppm
Gradient Suitability (at 254 nm): ≤ 1.0 mAU Water: ≤ 0.02%
Absorbance (at 210 nm): ≤ 0.699 AU Colour: Colourless
Absorbance (at 225 nm): ≤ 0.170 AU Acidity: ≤ 0.2 µeq/g
Absorbance (at 254 nm): ≤ 0.013 AU Alkalinity: ≤ 0.2 µeq/g

 

HPLC Water Specifications:

Gradient Suitability (at 210 nm): ≤ 5 mAU Al (Aluminium): ≤ 10 ppb
Gradient Suitability (at 254 nm): ≤ 0.5 mAU Ca (Calcium): ≤ 100 ppb
Absorbance (at 210 nm): ≤ 0.020 AU Fe (Iron): ≤ 5 ppb
Fluorescence (as quinine at 245 nm): ≤ 1.0 ppb K (Potassium): ≤ 10 ppb
Fluorescence (as quinine at 365 nm): ≤ 0.5 ppb Mg (Magnesium): ≤ 20 ppb
Residue on Evaporation: ≤ 5 ppm Na (Sodium): ≤ 200 ppb
Total Organic Carbon (TOC): ≤ 30 ppb Other Metals (ICP-MS): Suitable for LC analysis
  Cl- (Chloride): ≤ 10 ppb
  NO3- (Nitrate): ≤ 10 ppb 
  SO42- (Sulfate): ≤ 10 ppb
  PO43- (Phosphate): ≤ 10 ppb

 

Literature

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Agilent InfinityLab solvents for HPLC and LC/MS
Agilent InfinityLab Gradient Grade HPLC Solvents