Agilent SUPREMA Aqueous SEC Columns
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Agilent SUPREMA columns offer an optimal solution for aqueous SEC analysis of both neutral and anionic polymers, with typical applications including PEO, PEG, pullulan, dextran, polyacrylamide, hyaluronic acid, polyacrylic acid, and carboxymethyl cellulose. These columns, based on acrylate copolymer, come in three particle sizes (3, 5, and 10 μm), with various porosities ranging from 30 to 30,000 Å and options for linear/mixed-bed configurations, to address application requirements across a molecular weight range of 100 to 30,000,000 Da. Additionally, SUPREMA columns meet USP L25, L37, L38, and L39 designations, show high tolerance to high buffer concentrations, temperatures up to 80°C, organic solvents (up to 100% methanol or acetonitrile), and pH range of 2 to 12.
Features
- SUPREMA columns are packed with highly porous particles of a modified acrylate copolymer
- Recommended for aqueous SEC separations of neutral and anionic polymers
- Compatible with a wide range of aqueous eluents with optimum performance when used with eluents that have a pH in the range 6.0 to 10.2
- Meets USP L25, L37, L38, and L39 classifications
- Shipping solvent is water with 7.5 mM sodium azide to prevent microbial growth
- Flexible configurations available in three particle sizes, 3, 5, and 10 μm, and various individual porosities, between 30, and 30,000 Å as well as different linear/mixed-bed columns
- Excellent separation efficiency obtained by narrow particle size distribution and optimised frit technology
- Columns manufactured under DIN EN ISO 9001 quality standards
- Individually tested columns with a certificate of analysis available
- Guard columns protect and extend the lifetime of analytical columns
- SUPREMA Lux is recommended for light scattering detection
Specification
| SUPREMA | |||||||||||
| Pore Size | 30 Å | 100 Å | 300 Å | 1000 Å | 3000 Å | 10,000 Å | 30,000 Å | Mixed S | Mixed M | Mixed XL | Mixed Ultrahigh |
| Particle Type | Individual Pore Size | Individual Pore Size | Individual Pore Size | Individual Pore Size | Individual Pore Size | Individual Pore Size | Individual Pore Size | Mixed Pore Size | Mixed Pore Size | Mixed Pore Size | Mixed Pore Size |
| Molecular Weight Lower Limit | 100 Da | 100 Da | 1000 Da | 1000 Da | 10,000 Da | 10,000 Da | 50,000 Da | 100 Da | 200 Da | 200 Da | 500 Da |
| Molecular Weight Upper Limit | 30,000 Da | 100,000 Da | 300,000 Da | 1,000,000 Da | 3,000,000 Da | 10,000,000 Da | 30,000,000 Da | 100,000 Da | 1,000,000 Da | 3,000,000 Da | 30,000,000 Da |
| Particle Size | 3 µm, 5 µm, 10 µm | 3 µm, 5 µm, 10 µm | 10 µm | 3 µm, 5 µm, 10 µm | 10 µm | 10 µm | 10 µm | 5 µm | 5 µm, 10 µm | 10 µm | 10 µm |
| Temperature Limit | 80°C | 80°C | 80°C | 80°C | 80°C | 80°C | 80°C | 80°C | 80°C | 80°C | 80°C |
| Shipping Solvent | Water | Water | Water | Water | Water | Water | Water | Water | Water | Water | Water |
