HPLC TROUBLESHOOTING
1 DAY - IN PERSON AT YOUR LAB
OR 2 HALF DAYS - LIVE ONLINE
Resolving a problem with HPLC is usually straightforward. Finding the root cause of the problem within the complex environment of an HPLC analysis is much more challenging. This training seeks to develop a process driven approach to troubleshooting the entire holistic process, allowing delegates to develop skills to be able to tackle any problems.
These skills are applied within a strongly workshop driven environment, where we use many of the most common issues seen with HPLC analysis to consolidate the taught practices.
This is a challenging module and delegates should already have a good understanding of the HPLC process and associated instrumentation.
HPLC TROUBLESHOOTING COURSE OVERVIEW
Who is this course for?
This course is designed for those relatively new to LC and ideally will follow-on from attendance to the Fundamental LC training course.
Previous Knowledge
Some previous knowledge of gas chromatography and basic experience are recommended but not essential.
What you will learn
- How the chromatographic process takes place
- The architecture of a LC system
- Working principles and problems associated with each component of the system
- How to use chromatograms to troubleshoot chromatographic problems
- Best practices for column use and care
- Best practice in system maintenance and performance evaluation
Related Content
HPLC TROUBLESHOOTING COURSE OUTLINE
The Troubleshooting Process
- Getting the full picture
- Instrument benchmarking
Precisions Issues
- Troubleshooting Contamination Issues
- Extra peaks
- Carryover
- Baseline disturbances
Troubleshooting Column Related Problems
- Physical column damage
- Pressure issues
- Fronting / tailing peaks
- Split peaks / shoulders
- Preventing column issues
Sensitivity Issues
- High noise
- Low signal
Overview of Tackling Method Related Issues
- Tackling Issues Related to Analyst Error
- Common errors during preparation
- Calculation errors