LC-MS FOR PROTEINS AND PEPTIDES - INTACT MOLECULE
TRAINING COURSE
As biotherapeutics, enzymes, and peptide-based modalities continue to grow, so does the need for analysts who understand how to acquire and interpret high-quality LC-MS data. LC-MS for Proteins and Peptides - Intact Molecule provides a practical, structured introduction to protein and peptide mass analysis - from core molecular principles through advanced acquisition and deconvolution techniques.
Whether you're supporting biopharmaceutical development, working in proteomics, or contract research, this course equips you with the foundational tools to interpret the unique and complex spectra generated from protein and peptide applications. This material is also useful to analysts requiring a better fundamental understanding of software derived interpreted results.
The material is carefully designed with extensive embedded workshop content to practice skills as they develop during the training.
This course is ideal for analysts, bioanalytical scientists, and researchers working with proteins, peptides, or biotherapeutics who want a clear, practical grounding in intact-mass LC-MS.
LC-MS FOR PROTEINS AND PEPTIDES - INTACT MOLECULE COURSE DETAILS
- Protein Structure Fundamentals: Amino acids, solution chemistry, and how peptides and proteins form and behave in MS
- Intact Protein Chromatography: How to choose columns and optimise mobile phases for clean, MS-ready separations
- Carbon-13 Isotopes: How isotopic abundance, A+n contributions, and HRAM resolution affect intact-mass analysis
- Multiple Charging: m/z principles, A+1 delta, and deconvolution of low-resolution spectra. Interpreting high-mass A+n patterns and deconvoluting large molecules using HRAM data
- Peptide Digests: Enzymatic digestion workflows, tryptic and alternative fragments, and HPLC analysis of peptide mixtures
- Data-Dependent Acquisition: How DDA identifies and characterizes peptides in non-targeted workflows
- In-Source CID: Understanding early fragmentation and its impact on intact and subunit analysis
We offer flexible delivery options for this instructor-led course:
- On-site: One full-day session at your laboratory or any location of your choice.
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Online: Two half-day interactive sessions.
View our online training calendar for upcoming dates.
Experienced in small molecule LC-MS.
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Foundation – Protein Structure
- Amino acids
- Solution chemistry
- Peptides
- Proteins
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Intact Protein Chromatography
- Column selection
- Mobile phase conditions
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Carbon 13 Isotopes
- HRAM instruments
- Isotopic abundance
- Contribution at A+n of high molecular weight compounds
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Multiple Charging (1)
- Explanation of m/z
- A+1 delta
- Deconvolution of low resolution spectra
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Multiple Charging (2)
- Combining high molecular weight A+n with multiple charging
- Deconvolution of high molecular weight compounds from HRAM spectra using A+n delta
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Peptide Digests
- Enzymatic subunit cleavage
- Peptide digestion process
- Peptide fragments formed from trypsin digestion
- Alternative proteolytic enzymes
- HPLC analysis of peptides - Column selection - Mobile phase composition
- Workshops
- Data Dependent (Non-targeted) Acquisition
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In-Source Collision Induced Dissociation
