HPLC METHOD DEVELOPMENT
TRAINING COURSE
This instructor-led course provides a comprehensive, process-driven approach to the development of an optimal and robust HPLC method.
For the experienced chromatographer, this course provides a detailed exploration of each critical parameter within a typical method, then builds this into a step-by-step process for method development. We apply relevant theory to underpin a practical approach that maps out the optimal route to a commercial quality method. Importantly, we look at building robustness into each stage of development using a Quality by Design approach, highlighting many common problems and how we develop these out.
HPLC METHOD DEVELOPMENT COURSE DETAILS
- Setting method development objectives
- Sample preparation
- System choices
- Choosing a column and mobile phases
- Optimising strategies
- Quantitation and system characterisation
We offer flexible delivery options for this instructor-led course:
- On-site: One full-day session at your laboratory or any location of your choice.
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Online: Two half-day interactive sessions.
View our online training calendar for upcoming dates.
Delegates should have a good knowledge of chromatography and experience as HPLC users. Some experience in method development is recommended. A good grounding in chemistry is also beneficial.
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Objectives
- Design specification
- What is QBD?
- Building in robustness
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Influencing Resolution
- Retention factor
- Efficiency
- Selectivity
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Sample Preparation
- Influencing sample solubility
- Choosing a diluent
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Mobile Phase
- Sample polarity
- Organic modifiers
- pH control
- Flowrates
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Columns
- Physical dimensions
- Particle type, sizes and UHPLC
- Temperature
- Chemically modified silica
- Reversed phase ligands
- Hydrophobic subtraction model classification
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Gradient Elution
- Isocratic or gradient
- Optimising k’
- Effect of each gradient parameter
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Building a Practical Process
- Sample preparation
- Retention
- Screening for alpha
- Chromatographic optimisation
- Linearity, range and sensitivity
- Pre-validation
HPLC METHOD DEVELOPEMENT COURSE OVERVIEW
Course Overview
Course Walk-Through
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