AdvanceBio Peptide Plus HPLC Columns from Agilent

AdvanceBio Peptide Plus columns are reversed phase, superficially porous particle LC columns optimized for the separation of target peptides, impurities, and post translational modifications. These columns feature a hybrid endcapped C18 stationary phase on a 100 Å pore size, 2.7 µm particle modified to have a charged surface.

AdvanceBio Peptide Plus columns enable you to identify multiple critical quality attributes confidently by LC-MS. They provide the speed, sensitivity, and efficiency you need to quickly confirm and identify target proteins and peptides.

Agilent AdvanceBio Peptide Plus columns are high-performance reversed phase, superficially porous particle HPLC columns designed specifically for the analysis of peptides, peptide impurities, and post-translational modifications. Each Agilent Peptide Plus column features a hybrid endcapped C18 stationary phase with a 100 Å pore size and 2.7 μm particles modified with a charged surface. This unique surface modification enhances the selectivity and resolution of deamidated peptides relative to their unmodified counterparts, offering superior separation performance compared with standard C18 columns. 

Suitable for LC-MS applications, Agilent AdvanceBio Peptide Plus columns deliver the speed, sensitivity, and efficiency required to confidently identify multiple critical quality attributes in peptide mapping studies. Their superficially porous particle design reduces diffusion limitations, enabling high-efficiency separations of larger biomolecules at high linear velocities – without the backpressure typically associated with smaller fully porous particles. These characteristics make AdvanceBio Peptide Plus columns an excellent choice for laboratories performing detailed peptide analysis within the biopharmaceutical industry. 

For more demanding applications, the Altura Peptide Plus column provides a superior alternative. Based on the same advanced stationary phase as the Agilent Peptide Plus columns, it features an ultra-inert surface coating that effectively blocks active metal sites, resulting in improved peak shape, enhanced sensitivity, and greater sample recovery.