Agilent PROTEEMA SEC Columns
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Agilent PROTEEMA columns are designed for aqueous SEC separations of natural and synthetic proteins, peptides, and enzymes with molecular weights ranging from 100 to 7,500,000 Da. The columns feature a diol stationary phase that meets USP L20 specifications. Columns are available with a choice of porosities (100 Å, 300 Å, and 1000 Å), and two particle sizes (3 µm and 5 µm) to meet a variety of application needs. Bioinert hardware is offered to increase sensitivity and recovery for demanding applications, and PROTEEMA Lux columns are recommended for use with light scattering detectors.
Features
- Diol SEC chemistry that meets USP L20 classification
- Recommended for analysis of proteins, peptides and enzymes
- Compatible with a wide range of aqueous eluents with an optimum performance when used in pH range 2.0 to 7.5
- Shipped in water with 7.5 mM sodium azide to prevent microbial growth
- Narrow particle size distribution that contributes to excellent efficiency
- Excellent stability under a wide range of physical, chemical, and biological environments
- Guard columns protect and extend the lifetime of analytical columns
- Choice of stainless steel or bioinert hardware
- PROTEEMA Lux is recommended for use with light scattering detectors
Specifications
| PROTEEMA | |||
| Pore Size | 100 Å | 300 Å | 1000 Å |
| Bonded Phase | Diol | Diol | Diol |
| Particle Type | Fully Porous | Fully Porous | Fully Porous |
| Molecular Weight Lower Limit | 3 µm, 5 µm | 3 µm, 5 µm | 5 µm |
| Molecular Weight Upper Limit | 100 Da | 1000 Da | 1000 Da |
| Temperature Limit | 150,000 Da | 1,200,000 Da | 7,500,000 Da |
| pH Range | 2 - 8 | 2 - 8 | 2 - 8 |
| Shipping Solvent | Water | Water | Water |
| USP Designation | L20 | L20 | L20 |
Literature
Uncompromising analysis of polymers and macromolecules with the expanded portfolio of Agilent GPC/SEC columns and standards
Instructions and best practices for using the Agilent PROTEEMA SEC columns
What you should know when you need to analyze polymers, biopolymers, and proteins