Size Exclusion Chromatography (SEC) Columns
Size exclusion chromatography (SEC) columns are the ideal choice for monitoring aggregates of monoclonal antibodies. SEC is a routine technique for the analysis of proteins, and SEC columns are often used to evaluate the aggregation profile of a protein. Protein aggregates are a product of degradation and must be closely monitored and kept within acceptable limits. This is a Critical Quality Attribute (QCA) for monoclonal antibodies (mAbs) and other antibodies because it can cause an immunogenic response in patients.
Filter products
In SEC chromatography, molecules are separated according to their hydrodynamic radius rather than their absolute molecular weight, with larger species eluting before smaller ones. The pore size and volume of the stationary phase within the SEC column are critical to this isocratic separation process, making careful column selection vital for achieving optimal resolution and reproducibility. Typically, as the molecular weight of the biomolecule increases, a size exclusion column with larger pore size is selected. Element Lab Solutions offers a comprehensive range of SEC chromatography columns – from robust analytical SEC columns to advanced sub-2 µm and next-generation designs – designed to meet the evolving demands of modern biotherapeutic analysis.