HALO® BioClass OLIGO C18 Columns from Advanced Materials Technology
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HALO® BioClass OLIGO C18 columns are purpose-designed HPLC columns for oligonucleotides, combining the high efficiency of Fused-Core® particle technology with surface chemistries optimised for demanding oligonucleotide separations. Developed to deliver high resolving power, speed and robustness, HALO® Oligo columns support accurate characterisation of closely related impurities and failure sequences across a broad range of oligo lengths. Alkaline-resistant, surface-modified organo-silane chemistry and inert, surface-passivated column hardware ensure excellent stability, minimal adsorption and high analyte recovery under conditions commonly used for oligonucleotide analysis.
The HALO® 120 Å OLIGO C18 column is optimised for routine to advanced separations of short to mid-length oligonucleotides, typically up to 60 bases. Built on proven Fused-Core® technology, it delivers high efficiency and fast analysis while maintaining excellent performance at elevated pH and temperature. The stationary phase is fully compatible with UHPLC and mass spectrometry workflows, while the use of inert column hardware minimises potential interactions with stainless steel, supporting consistent, high-recovery separations.
For larger and more complex targets, the HALO® 1000 Å OLIGO C18 column extends performance to long-chain oligonucleotides, including sequences exceeding 100 nucleotides. The large pore structure enables improved mass transfer, higher peak capacity and superior impurity profiling compared with conventional columns, while maintaining lower backpressure to support longer column formats. Designed for use under high pH and elevated temperature conditions, this column is well suited to advanced applications such as therapeutic oligonucleotide development and CRISPR-related workflows, delivering reliable, high-resolution separations for the most challenging samples.
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Features
- Oligonucleotide-Optimised Design – Purpose-built stationary phase developed to meet the specific demands of oligonucleotide separations
- Optimised Pore Size – 120 Å pore size for oligomers up to 60 bases and 1000 Å pore size for high-efficiency separation of oligonucleotides up to and beyond 100 bases
- Fused-Core® Technology – Superficially porous particles deliver high efficiency, fast separations and low backpressure for improved analytical throughput
- High-Stability Performance – Designed for operation under high pH and elevated temperature conditions commonly used in oligonucleotide analysis
- UHPLC and LC-MS Compatible – Optimised stationary phase supports seamless integration with UHPLC systems and mass spectrometry detection
- Inert Column Hardware – Surface-passivated hardware minimises oligonucleotide adsorption, improving recovery, peak shape and reproducibility
- USP L1 Classified – Supports straightforward method development, transfer and regulatory alignment
Specification
| HALO® 120 Å OLIGO C18 | HALO® 1000 Å OLIGO C18 | |
| Phase | dimethyloctadecylsilane, surface modified | dimethyloctadecylsilane, surface modified |
| Particle Type | Fused-Core® | Fused-Core® |
| Particle Size | 2.7 µm | 2.7 µm |
| Pore Size | 120 Å | 1000 Å |
| Surface Area | 75 m2/g | 22 m2/g |
| Carbon Load | 5.60% | 2.4% |
| Endcapped | Yes | Yes |
| Low pH/Temperature Limit | 2/90⁰C | 2/90⁰C |
| High pH/Temperature Limit | 9/85⁰C | 9/85⁰C |
| Hardware | Inert | Inert |
| USP Designation | L1 | L1 |
Literature
Application Note 387 - Advantage of Fused-Core® Particle Technology for Oligonucleotide Separations
(478.41 kB)
Application Note 408 - Advantage of HALO 1000A over Competitor Columns for Oligonucleotides
(901.78 kB)
