HPLC TROUBLESHOOTING TRAINING COURSE
Resolving a problem with HPLC is usually straightforward. Finding the root cause of the problem within the complex environment of an HPLC analysis is much more challenging. This training seeks to develop a process driven approach to troubleshooting the entire holistic process, allowing delegates to develop skills to be able to tackle any problems.
These skills are applied within a strongly workshop driven environment, where we use many of the most common issues seen with HPLC analysis to consolidate the taught practices.
This is a challenging module and delegates should already have a good understanding of the HPLC process and associated instrumentation.
HPLC TROUBLESHOOTING COURSE DETAILS
- How the chromatographic process takes place
- The architecture of a LC system
- Working principles and problems associated with each component of the system
- How to use chromatograms to troubleshoot chromatographic problems
- Best practices for column use and care
- Best practice in system maintenance and performance evaluation
We offer flexible delivery options for this instructor-led course:
- On-site: One day at your lab or a location of your choice.
-
Online: Two half days.
View our online training calendar for upcoming dates.
Some previous knowledge of gas chromatography and basic experience are recommended but not essential.
- The Troubleshooting Process
- Getting the full picture
- Instrument benchmarking
-
Precisions Issues
- Troubleshooting Contamination Issues
- Extra peaks
- Carryover
- Baseline disturbances
-
Troubleshooting Column Related Problems
- Physical column damage
- Pressure issues
- Fronting / tailing peaks
- Split peaks / shoulders
- Preventing column issues
-
Sensitivity Issues
- High noise
- Low signal
-
Overview of Tackling Method Related Issues
- Tackling Issues Related to Analyst Error
- Common errors during preparation
- Calculation errors
