YMC Hydrosphere C18 HPLC Columns
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YMC Hydrosphere C18 Analytical HPLC Columns
The separation of polar compounds in many cases requires highly aqueous mobile phase conditions to achieve sufficient retention on the stationary phase. Conventional reversed phase selectivities do not give reproducible results under these conditions mainly due to the collapse of the C18 chains. Hydrosphere C18 has been developed, on the ultra pure silica support of the ProFamily, to be stable in 100% aqueous eluents.
For ultra-fast LC applications YMC-Ultra HT Hydrosphere C18 with 2 µm particle size provides the optimal selectivity for the separation of highly polar compounds.
The Hydrosphere C18 columns from YMC are manufactured with a “hydrophilic” C18 surface that is designed specifically to enhance the separation of polar compounds. Built on YMC’s ultra-pure, pH-neutral silica, these columns are tailored for pharmaceutical and biotechnology R&D, offering unparalleled performance in highly aqueous mobile phases.
Features
- C18 stationary phase that meets USP L1 specifications
- Superior retention of polar analytes
- Excels in the separation of hydrophilic compounds including peptides, nucleotides, and small polar molecules
- Maintains its structural integrity and reproducibility under 100% aqueous eluent conditions, preventing the collapse of C18 chains commonly seen in other columns
- Optimised to operate without the need for ion pair reagents, simplifying mobile phase preparation and improving method robustness
- Based on highly inert, ultra-pure fully porous silica
- Available in particle sizes of 2 µm, 3 µm, and 5 µm for flexibility in resolution and speed of analysis
- Guard columns protect the analytical column and help to extend its lifetime
- Range of dimensions available for seamless scale-up from microbore to preparative scale
Specifications
| Hydrosphere C18 | |
| Bonded Phase | C18 |
| Particle Type | Fully Porous |
| Particle Size | 2 µm, 3 µm, 5 µm |
| Pore Size | 120 Å |
| Surface Area | 330 m2/g |
| Carbon Load | 12% |
| Maximum Temperature | 50°C |
| AQ Stability | 100% aqueous eluents |
| pH Range | 2 - 8 |
| Hardware | Stainless Steel |
| USP Designation | L1 |
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