AdvanceBio SEC Columns from Agilent
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AdvanceBio SEC columns from Agilent are high-performance size exclusion chromatography (SEC) columns that provide precise, reproducible quantitation of aggregates and fragments in peptides, monoclonal antibodies (mAbs), and larger biotherapeutics. Designed for robust, reliable performance, these Agilent SEC columns deliver high-resolution separations across a broad molecular weight range, from small proteins to viral particles, enhancing laboratory efficiency, supporting method transfer, and reducing analytical uncertainty across columns, batches, and sites.
SEC Columns for small therapeutic proteins and peptides:
- AdvanceBio SEC 130 Å, 2.7 μm: Routine aggregate analysis (100 Da–120 kDa)
- AdvanceBio SEC 120 Å, 1.9 μm: High-resolution, rapid profiling (1–80 kDa)
SEC Columns for monoclonal antibodies and ADCs:
- AdvanceBio SEC 300 Å, 2.7 μm: General aggregate screening (1 kDa–1.25 MDa)
- AdvanceBio SEC 200 Å, 1.9 μm: mAb fragment analysis (2–700 kDa)
SEC Columns for AAVs and VLPs:
- AdvanceBio SEC 500 Å, 2.7 μm: Suitable for AAVs (5 kDa–5 MDa or Rh 3–25 nm)
- AdvanceBio SEC 1000 Å, 2.7 μm: Ideal for larger VLPs (15 kDa–10 MDa or Rh 5–40 nm)
Features
- High resolution, high-efficiency separation, and accurate quantitation for aggregates and fragments analysis
- Prevented secondary interactions with mAb, ADC, and other proteins
- Assured consistent column performance to eliminate rework
- Enhanced column lifetime and productivity to meet robust CQA analysis
- Specially designed standards for AdvanceBio SEC columns, providing optimal calibration and performance verification
- AdvanceBio SEC 300 Å 2.7 µm is intended for separation of proteins ranging from 5,000 to 1.25 million Da including mAb fragments, mAb monomer, dimer, and high-level aggregates
- AdvanceBio SEC 130 Å 2.7 µm is designed for small therapeutics with molecular weight ranging from 100 to 120,000 Da
- AdvanceBio SEC 500 Å is recommended for aggregate analysis of large biomolecules such as AAVs and VLPs in the range 5000 Da - 5 MDa
- AdvanceBio SEC 1000 Å is designed for analysis of large biological molecules such as VLPs and large oligonucleotides with molecular weight range of 15,000 Da to 10 MDa
- AdvanceBio SEC 200 Å 1.9 µm is suited for analysing protein ranging from 2,000 to 700,000 Da including mAb monomer, dimmer, and LMW protein fragments
- AdvanceBio SEC 120 Å 1.9 µm is best suited for analysis of peptides and small therapeutic proteins in the range 100 to 80,000 Da
Specifications
| AdvanceBio SEC | ||||||
| Pore Size | 120 Å | 130 Å | 200 Å | 300 Å | 500 Å | 1000 Å |
| Particle size | 1.9 µm | 2.7 µm | 1.9 µm | 2.7 µm | 2.7 µm | 2.7 µm |
| Particle Type | Fully Porous | Fully Porous | Fully Porous | Fully Porous | Fully Porous | Fully Porous |
| Molecular Weight Lower Limit | 1000 Da | 100 Da | 2000 Da | 5000 Da | 5000 Da | 15,000 Da |
| Molecular Weight Upper Limit | 80,000 Da | 120,000 Da | 700,000 Da | 1,250,000 Da | 5,000,000 Da | 10,000,000 Da |
| Temperature Limit | 80°C | 80°C | 80°C | 80°C | 80°C | 80°C |
| pH range | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 |
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Literature
Accurately determine biomolecule aggregation and fragmentation with AdvanceBio SEC columns
End uncertainty in monoclonal antibody characterization with AdvanceBio 2.7 µm columns
High-resolution analysis of peptides and proteins with AdvanceBio SEC 120 Å 1.9 µm columns
Fast separations for aggregates and fragments with AdvanceBio SEC 200 Å 1.9 µm columns
High-speed, high-resolution aggregate analysis of ultra-large biotherapeutics
Collection of Agilent app notes on aggregate/fragment analysis
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