Advion-Interchim puriFlash 5.250

The puriFlash 5.250 flash system from Advion-Interchim Scientific is a compact yet highly versatile purification system designed to meet a wide range of chromatographic requirements, from routine separations to complex, high-sensitivity purifications. Supporting both normal and reverse phase applications, as well as flash chromatography and preparative HPLC, it offers a flexible platform well-suited to synthetic, analytical, and process development laboratories.

With a maximum flow rate of 250 mL/min and an operating pressure of up to 250 bar, the system is capable of handling both low- and high-complexity samples efficiently. This makes it particularly valuable for tasks such as impurity profiling, trace enrichment, and the purification of minor components.

The puriFlash 5.250 incorporates triple detection capability – UV, ELSD, and MS – to ensure accurate identification and recovery of target compounds, including those present at low concentrations. The enhanced detection sensitivity supports comprehensive fraction collection, reducing the risk of overlooking critical components.

Designed for continuous operation with minimal maintenance, the puriFlash 5.250 includes built-in safeguards such as RFID component recognition and leak detection to enhance safety and reduce setup errors. System control is handled via a 15″ touchscreen with ClearPeak software and Genius automation, enabling remote access, method transfer, and purification setup based on TLC or analytical data – streamlining workflows and improving overall efficiency.

Combining performance, adaptability, and intelligent automation, the puriFlash 5.250 is a robust solution for laboratories requiring dependable, high-throughput purification across a diverse range of applications.

Features

  • ClearPeak Software Control – Comprehensive system management with an intuitive interface for method setup and monitoring
  • TLC to Flash & Prep App Compatibility – Enables direct method transfer from TLC analysis to flash or preparative chromatography via smartphone integration
  • 15″ Touchscreen with Remote Monitoring – Large display for real-time control and system oversight, locally or remotely
  • UV Detection (200–400 nm) – Multi-wavelength and collective scan functionality for precise, flexible compound detection
  • Wide Column Compatibility – Supports both flash and preparative columns with inner diameters up to 80 mm
  • High-Capacity Rack System – Accommodates 3 long racks and up to 132 tubes for extended fraction collection
  • Maximum Flow Rate – 250 mL/min for high-throughput purification
  • Maximum Operating Pressure – 250 bar for use with high-efficiency columns and complex separations
  • Upgradeable Performance Packs – Optional ‘UVextended’, ‘iELSD’, ‘Multi’, and ‘PWD’ modules available to enhance detection and purification capabilities

Specification

  puriFlash 5.250
Pump  
Flow rate 250 mL/min
Max pressure 250 bar
Quaternary gradient yes
Air purge yes
Washing discs yes
Pump washing discs (pack) pack-PWD
   
Detector  
UV: 200 - 400 nm multi wavelength & scan collection yes
UV: 200 - 800 nm multi wavelength & scan collection pack-UVextended
Spectral view and purity confirmation yes
iELSD Detection (pack) pack-iELSD
   
Injection  
4 port electrical valve no
6 port electrical valve with loop no
6 port + 10 port electrical valves with loop yes
Injection mode: liquid - dry-load yes
   
Column Selection Valve  
6 port electrical valve pack-Multi
   
System Optimisation  
Tubing after pump 1/8" x 1.6 mm ID
Flow cell: optical length/volume 0.3 mm/40 µL
   
Column Holder  
Integrated yes
Pre-column holder yes
   
Fraction Collector  
Reglular collector 3 racks Gen5
132 tubes 18 x 150 mm
   
Unit Control  
Touch screen 15" yes
USB 8
RJ45 yes
   
Safety  
Leak detection (pump, FC, iELSD, holders) yes
Solvent tray with drainage system yes
Collector with drainage system yes
Solvent and waste level monitoring yes
RFID yes
Fume enclosure yes
   
Size Width: 40 cm
Depth: 51 cm
Height: 75 cm

Literature

The essential systems and consumables for purification, evaporation, and mass spectrometry
puriFlash 5.250 - optimised for your preparative purifications

Videos