Uptisphere Strategy Prep HPLC Columns from Advion-Interchim Scientific

All pictures shown are for illustration purpose only. Actual product may vary.

Uptisphere Strategy preparative HPLC columns from Advion-Interchim Scientific are designed for high-efficiency identification, quantification, and purification of small molecules and pharmaceutical compounds. Built on high surface area silica and available in internal diameters from 4.6 mm to 50 mm with 5 µm, 10 µm, or 15 µm particle size, they deliver strong mechanical stability and reliable performance across a wide range of chromatographic modes.

Uptisphere Strategy prep columns are available in different phase options tailored to a range of separation needs.

Uptisphere Strategy C18-3: Designed for strong retention of non-polar analytes, this densely bonded and fully end-capped C18 phase delivers reliable performance under basic conditions and supports the purification of basic drugs up to pH 12.
Uptisphere Strategy C18-HQ: Offering high loading capacity from its large surface area, this versatile reversed phase C18 material provides robust retention and broad applicability across pharmaceutical purification workflows.
Uptisphere Strategy C18-RP: Developed for mid- to non-polar compounds, this mechanically stable reversed phase material maintains consistent selectivity and performance under both acidic and basic preparative conditions.
Uptisphere Strategy PHC4: Optimised for analytes containing aromatic rings or exhibiting mid-polarity, this phase delivers enhanced π-selective interactions for improved resolution of structurally complex compounds.
Uptisphere Strategy HILIC-HIT: Suitable for highly polar, water-soluble analytes, this hydrophilic interaction phase supports aqueous normal phase separations and offers an effective alternative to reversed phase purification for compounds with limited RP retention.
Uptisphere Strategy HILIC-HIA: Tailored for very polar molecules requiring strong HILIC retention, this phase operates with water/acetonitrile mobile phase and provides enhanced separation when reversed phase modes are insufficient.
Uptisphere Strategy SI: A traditional silica phase suited for polar organic compounds, offering straightforward normal phase retention with minimal surface modification for applications requiring simple, non-ionic interactions.

Buy Features Specification Literature Related Products

		Part Number	Product Description	Price	Qty

Features

Comprehensive Phase Selection – Provides tailored options for reversed phase, HILIC, and normal phase purification workflows
High Surface Area Silica – Supports greater loading capacity and improved preparative efficiency
Robust Mechanical Stability – Developed to withstand demanding flow rates and pressure conditions
Versatile Particle Sizes – Available in 5 µm, 10 µm, and 15 µm formats to match method and scale requirements
Wide pH Compatibility – Delivers reliable performance across acidic to strongly basic environments
Scalable Column Dimensions – Offered from 4.6 mm to 50 mm ID for seamless transition from analysis to purification
Extended Column Lifetime – Guard cartridges, when used with the appropriate guard holder, provide extra protection to maximise the life of your prep column

Specification

	C18-3	C18-HQ	C18-RP	PHC4	HILIC-HIT	HILIC-HIA	SI
Phase	C18	C18	C18	Phenyl-Butyl	Proprietary	Proprietary	Silica
Mode	Reversed Phase	Reversed Phase	Reversed Phase	Reversed Phase	HILIC	HILIC	Normal Phase
Particle Type	Fully Porous	Fully Porous	Fully Porous	Fully Porous	Fully Porous	Fully Porous	Fully Porous
Particle Size	5 µm, 10 µm, 15 µm	5 µm, 10 µm, 15 µm	5 µm, 10 µm, 15 µm	5 µm, 10 µm, 15 µm	5 µm, 10 µm, 15 µm	5 µm, 10 µm, 15 µm	5 µm, 10 µm
Pore Size	100 Å	100 Å	100 Å	100 Å	100 Å	100 Å	100 Å
Surface Area	425 m2/g	425 m2/g	425 m2/g	300 m2/g	425 m2/g	300 m2/g	425 m2/g
Carbon Load	22%	19%	16%	12%
Endcapped	Multi step	Multi step	Mixed Multi step	One step	Proprietary	Proprietary	No
pH Range	1 - 12	1 - 10	1.5 - 8	1.5 - 7.5	1.5 - 7.0	2 - 7	1.5 - 7