HPLC FOR BIOMOLECULES TRAINING COURSE
This intensive course provides an extensive overview of the use of HPLC in the analytical support of biomolecular proteins, particularly monoclonal antibodies (mAbs).
We provide a foundation in understanding proteins and an introduction on how they are produced using biotechnology. We then look at specific HPLC techniques used in the analysis of amino acids, peptides, intact and subunit proteins. This includes size exclusion chromatography, ion exchange chromatography, reversed phase chromatography, HILIC and HIC.
It is of particular use to delegates from a small molecule analytical background looking to apply their experience to the analysis of biomolecules.
Please note that this training does not cover LC-MS.
HPLC FOR BIOMOLECULES COURSE DETAILS
- Overview of the different types of biomolecules
- Biotechnology and the production of proteins
- SEC and aggregates
- IEX for charge variancy
- Reversed phase for multiple applications
- HILIC and HIC
We offer flexible delivery options for this instructor-led course:
- On-site: One full-day session at your lab.
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Online: Two half-day interactive sessions.
View our online training calendar for upcoming dates.
Delegates should already have a good understanding of HPLC.
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Biomolecules
- Amino Acids and Peptides
- Primary, secondary, tertiary and quaternary protein structure
- Post translational modification
- Monoclonal antibodies
- Antibody drug conjugates (ADC)
- Oligonucleotides
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Introduction to Biotechnology
- Nucleotides, DNA, mRNA, tRNA
- Transcription and translation
- ICH Q6(B)
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Size Exclusion Chromatography
- Protein aggregation
- SEC columns and mobile phases
- Basic method development
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Ion Exchange Chromatography
- Charge variancy
- IEX modes
- pH and salt gradient
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Reversed Phase Chromatography
- Columns and mobile phases
- Protein primary structure
- Peptides
- Derivatised amino acid analysis
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HILIC
- Overview of technique
- Glycan analysis
- Un-derivatised amino acid analysis
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Hydrophobic Interaction Chromatography (HIC)
- Overview of technique
- Common uses
